A rapid method that uses small volumes of isolate culture to resolve specific enzyme activity is needed. However, the growth temperature and conditions that yield high cellulase activity vary widely, and the throughput to identify optimal conditions has been limited by the slow handling and conventional analysis. Microbial communities are known to express a wide range of enzymes necessary for the saccharification of lignocellulosic feedstocks and serve as a powerful reservoir for enzyme discovery. more Production of biofuels via enzymatic hydrolysis of complex plant polysaccharides is a subject of intense global interest. Production of biofuels via enzymatic hydrolysis of complex plant polysaccharides is a subject of. The observation of 2 Fe(4)S(4) clusters for CooF contradicts the prediction of 4 Fe(4)S(4) clusters based on analysis of the amino acid sequence of CooF and structural studies of CooF homologs. Metal analysis and EPR spectroscopy show that CooF contains 2 Fe(4)S(4) clusters. The characteristic EPR signals observed for CODH are largely insensitive to CooF complexation. Here we present the spectroscopic and biochemical properties of the CODH:CooF complex. CooF is an Fe-S protein that is the proposed mediator of electron transfer between CODH and the CO-induced hydrogenase. However the electron transfer pathway from CODH to the CO-induced hydrogenase that evolves H(2) is not well understood. The CO-oxidizing enzyme, carbon monoxide dehydrogenase (CODH), has been purified and extensively characterized. more Upon exposure to CO during anaerobic growth, the purple phototrophic bacterium Rhodospirillum rubrum expresses a CO-oxidizing H(2) evolving enzymatic system. Upon exposure to CO during anaerobic growth, the purple phototrophic bacterium Rhodospirillum rub. Purification and detailed characterization of these proteins has afforded new insight into the possible mechanism of Fe(II) oxidation by Leptospirillum Group II, the dominant population in most of these biofilms, and demonstrated that the abundance and posttranslational modifications of one of these proteins is dependent on the lifecycle of the biofilm. Here we report the isolation of two abundant heme proteins from low-diversity biofilm microbial communities that thrive in very acidic (pH ~ 1), metal-rich water in a subsurface mine. Recent advances in genomic sequencing and proteomic measurements of samples obtained from natural communities have allowed new access to these uncultivated extremophiles and identified abundant proteins that can be isolated directly from natural samples. more Microorganisms from extreme environments are often very difficult to cultivate, precluding detailed study by biochemical and physiological techniques. Microorganisms from extreme environments are often very difficult to cultivate, precluding detail. Although there are subtle differences in potentially lignocellulolytic taxa between GPC- and GWC-derived communities. However, the effect of compost source was significantly less than the effects of the other culture variables. The enriched community structure and respiration were influenced by the choice of feedstock, compost type, and application of thermophilic, high-solids conditions. Respiration was monitored as a measure of microbial activity, and 16S ribosomal RNA gene sequencing was used to characterize microbial community structure. In this study, microbial communities from green waste compost (GWC) and grape pomace compost (GPC) were cultured on switchgrass and eucalyptus to observe the impact of inoculation on feedstock decomposition and microbial community structure. more Culturing compost-derived microbial communities on biofuel feedstocks under industrial conditions is a technique to enrich for organisms and lignocellulolytic enzymes for bioenergy feedstock deconstruction. Culturing compost-derived microbial communities on biofuel feedstocks under industrial conditions.
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